Non‐specific phospholipases C2 and C6 redundantly function in pollen tube growth via triacylglycerol production in Arabidopsis

Non-specific phospholipase Cs (NPCs) are responsible for membrane lipid remodeling that involves hydrolysis of the polar head group phospholipids. Arabidopsis NPC2 and NPC6 essential in gametogenesis, but their underlying role remains elusive. Here, we show these NPCs required triacylglycerol (TAG) production pollen tube growth. highly expressed developing tubes localized at endoplasmic reticulum. Mutants showed reduced rate germination, length amount droplets (LDs). Overexpression or induced LD accumulation, which suggests involved production. Furthermore, mutants defective biosynthesis TAG, a major component LDs, These results suggest gametogenesis hydrolyzing phospholipids producing TAG Thus, from to during growth represents an emerging NPC family plant developmental control.